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KMID : 0379119990270040299
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Korean Journal of Mycology
1999 Volume.27 No. 4 p.299 ~ p.303
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Purification of Phytase from Aspergillus ficuum and Production of Anti-phytase Antibody
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Abstract
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Phytase(myo-inositol-hexakis phosphate 3-phosphohydrolase, E C 3.1.3.8) sequentially hydrolyzes phytate to myo-inositol and inorganic phosphate. Phytase of Aspergillus ficuum was purified to homogeneity using ultrafiltration, ration exchange column and anion exchange column. It´s molecular weight is estimated as around 90,000 by SDS-PAGE. Antibody against the phytase was produced by immunizing mice with the purified phytase. The titer of the antibody was determined to be 1/25,000.
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